Collecting the desired cell sample from a cell culture is the very first step of studying and experimenting the cells. Dissociation is a necessary technique to target and isolate the interested cells. It sometimes called disaggregation which breaking down of a cell culture and result in a small group. Single-cell suspensions are obtained from the gauze filtration system in cell dissociation. A cell strainer is a faster, simpler alternative to gauze filtration when dissociating cells from clumps and producing more uniform single-cell suspensions.


Cell strainers remove clumps and debris from samples to isolate cells for further testing.  It can isolates the primary cells quickly and allow for a consistent and uniform single-cell suspension. The cell strainers are made of various sizes to allow certain materials to pass through. Unwanted material can be filtered out from the whole sample since the separation of the strainer based on the size of the materials. The common sizes of the cell strainers include 40µm and 70µm to help isolate cells of different sizes.

 

Advantages:

  • Easy to use
  • Rapid and sterile for the isolation of primary cells to obtain a uniform single-cell suspension consistently
  • Spores produced are evenly spaced, results are reliable and consistent.
  • Come in easily accessible packaging that is convenient and avoids contamination during storage.




Bel-Art FlowmiTM Disposable Cell Strainers for 1000µl pipette tips

Bel-Art Flowmi™ Cell Strainers provide fast and efficient filtering of small volume samples (up to 1000µl) prior to FLOW or FACS analysis. Patent pending.

 

  •          Preserves volume when filtering small sample volumes
  •          Removes cell aggregates and creates uniform single cell suspensions
  •          Decreases the potential for clogging of FLOW or FACS instruments by effectively filtering cellular debris
  •          Use with your favorite pipette tips; Flowmi™ Strainers fit most 1000µl tips including Fisherbrand Sure-One, VWR, and Eppendorf and more
  •          Compact tray holds 50 Flowmi™ Cell Strainers ready for press and fit attachment to your tip; features a sliding cover for easy, one-handed use
  •          Packaged in a re-sealable bag to maintain sterility when used in a laminar flow hood or biohazard hood
  •           Sterile, 40µm or 70µm porosity


       



Bel-Art FlowmiTM is recommended for use with samples having a maximum concentration of 2 million cells/ml (40µm) or 4 million cells/ml (70µm)






Please click here for more information.